Get to know your Zebrafish Embryos today
The Zebrafish Embryo Genotyping (ZEG) Unit and its accompanied ZEG chip comprise of a microfluidic system that enables genotyping of live zebrafish embryos by rapid collection of PCR amplifiable genetic material
“In the olden days, I would have had to sacrifice each embryo to genotype them, which really limited the types of experiments I could do. Now instead I have genotyped offspring at 48 hpf ready for experimentation, or to grow up, saving me valuable space in our zebrafish facility!”
Matthew Keefe, University of Utah
96 embryos per hour
>80% Sensitivity and >90% Survival
Compatibility
Gel electrophoresis, HRMA, Sequencing
Embryo Wellbeing
No observed adverse effects on survival, morphology, or Behavior
The Zebrafish Embryo Genotyper (The ZEG)
The ZEG is a great research tool that your Lab can use!
ZEG Chip Packs
Our 24 well ZEG Chips are disposable, PCR and Zebrafish safe!
Our Mission
wFluidx, Inc. provides simple, cost-effective, meaningful solutions in the Zebrafish Research Field.
Frequently Asked Questions
How do I increase the quality of the extracted DNA using PCR?
The polymerase/mix you use is critical. Best results are obtained using Phire Polymerase, #F126 from Thermo-Fisher, an amplicon smaller than 300bp and 40 cycles of PCR.
How do I increase the quality of the extracted DNA post-PCR using HRMA?
For HRMA, the best results use LC Green from Bio Fire (you need to optimize the annealing temp; it is usually a bit higher) or Bio Rad’s HRMA master mix.
How do I increase the concentration of the extracted DNA using PCR?
To increase the concentration extracted we suggest not to dilute or do a Thermopol isolation on the ZEG samples at all, use 5 ul of ZEG material directly in the PCR mix.
Are chips Reusable?
It is not recommended to reuse chips; this will lead to cross contamination if not cleaned properly.
Do I have to optimize my PCR before using the ZEG?
We recommend optimizing your PCR procedure before you use the ZEG. Design primers that amplify more robustly and reliably on a fin clip, and then try them on ZEG.
Can I use the ZEG on unhatched Zebrafish Embryos?
Fish inside the chorion won’t work. If you must do ZEG before they hatch, manually dechorionated them first, or wait until they hatch.
Do I need to use mineral oil for PCR or HRMA?
You don’t need mineral oil for PCR but you do need it for HRMA.
How many PCR samples can I get from one run?
If you use 15ul for each embryo on the chip, you get back at least ~12ul. You could do potentially 3 PCRs with that, using 4ul each.
What is the voltage on the ZEG supposed to be set too?
All ZEG units manufactured and sent since July 2022 are outside the range ZP-001 and ZP-041, so the voltage should be set to 1.8V. Those sent earlier to 1.4 V.
How do I change the ZEG voltage?
If you need to change the voltage:
- The base unit controller voltage can be changed from 1.4 V to 2.4 V by the following method. PLEASE NOTE: Do not set the voltage to or beyond 3V. This will damage the vibration motor.
- Press the yellow SET button. This would highlight the ‘SET’ word on the LED screen.
- Press in the silver knob several times until the ‘1’ of the ’01.80’ (on the LED screen) is highlighted.
- Rotate the knob clockwise (to increase) or counter-clockwise (to decrease) this value. Change this value from ‘1’ to ‘2’, until desired voltage is set.
- Press in the silver knob.
- Press the yellow SET button until the screen returns to original screen.
Is it possible to add anesthesia to the extraction water without having any impact on the results?
Tricaine (the typical fish anesthesia) doesn’t affect ZEG results in any way.
Is the cover enough to avoid evaporation or are there any extra precautions we should follow?
There aren’t any extra precautions you need to take to avoid evaporation, the cover provided with the ZEG is enough.
I have faulty chips, what do I do?
Please contact customer support by emailing info@wfluidx.com
My ZEG stopped working, what do I do?
Please contact customer support by emailing info@wfluidx.com
President/Founder
CHRISTOPHER LAMBERT
Chief Executive Officer
MADISON HANSEN
Manufacturer
CHELSEY HANSEN
Let’s work together and find meaningful solutions in the Zebrafish Research Community
Our product will allow Zebrafish research labs to save time, funding, facility space, and allow them to know their fish at earlier stages of development!