Q: What are the power specifications for ZEG?
A: The converter included with the unit will allow you to use an AC power input ranging between 100 - 200 V, 50/60 HzThe converter output is DC and has an output of 12 V and 2 A. That amount is regulated to 1.4 V and 0.03 A by the controller housed in the base unit.
Q: Does changing the extraction time increase sensitivity?
A: It depends. Users have reported that both increases and decreases in extraction time have yielded improved genotyping efficiency. We encourage you to figure out what works best for your group-- and let us know if any changes make a big impact!
Q: How can I increase genotyping sensitivity?
A: We recommend using fresh primers solutions and a hot start polymerase or a direct from tissue PCR kit. Make sure you are getting the most volume you can off of each well after the extraction is completed, which should be 10 µL or more. Extending the number of PCR cycles may also aid in increasing sensitivity.
Q: I’m getting impossible genotypes when I’m using ZEG, why is this happening?
A: Since ZEG is used for live zebrafish embryos, the amount of extracted DNA is going to be smaller than a tissue sample. Getting desired or acceptable results might require tweaking of PCR protocols, extended cycling, fresh primers, etc.
Q: How big of gene fragments can I genotype with ZEG extracted DNA?
A: We recommend aiming for 250 bp or smaller gene fragments. Anything over that and you may experience decreased sensitivity or will have to modify your PCR protocols or chemistry. We are currently working to improve ZEG’s sensitivity for larger amplicons.
Q: Can I use chorionated embryos?
A: No. ZEG requires dechorionated embryos.
Q: How old of embryos/larvae can I use with ZEG?
A: ZEG was developed to be used with 72 hpf embryos. It has been reported to us that 24 hpf and 48 hpf embryos can be used with ZEG with >90% sensitivity and survivability. The embryos must be dechorionated first, though.
Frequently Asked Questions
User Manual and Video Tutorial
Download ZEG User Manuel Here
ZEG Video Tutorial